FastQCFastQC Report
Wed 5 Jul 2017
FCHGVKNBBXX-HKZEBggcRAAARAAPEI-201_L3_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameFCHGVKNBBXX-HKZEBggcRAAARAAPEI-201_L3_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26623689
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCATTCCTCGCCTCTGCTTCGAGAACAGTGTGACCATGGCAGTGTGGC1007040.3782496107132261No Hit
ACCATATCCACCATTCCTCGCCTCTGCTTCGAGAACAGTGTGACCATGGC653200.24534541400329607No Hit
CCTCGCCTCTGCTTCGAGAACAGTGTGACCATGGCAGTGTGGCTTCAGGC624440.2345430041644492No Hit
CTCGCCTCTGCTTCGAGAACAGTGTGACCATGGCAGTGTGGCTTCAGGCT620240.23296546169841453No Hit
ATCCACCATTCCTCGCCTCTGCTTCGAGAACAGTGTGACCATGGCAGTGT576130.21639750975155997No Hit
CTTCAGGCTGGTGCTCTGTTGGTCCTGTTGGTCGTGTCCAGTGTAAGCAC433760.16292257620647538No Hit
CCACCATTCCTCGCCTCTGCTTCGAGAACAGTGTGACCATGGCAGTGTGG391840.14717719997405318No Hit
CCATTCCTCGCCTCTGCTTCGAGAACAGTGTGACCATGGCAGTGTGGCTT342500.12864483205163643No Hit
CTGGTCGATGCCCTTTATCTGGTCTGTGGCCCAACAGGCTTCTTCTACAA327790.12311967736702455No Hit
CTGGCATTGGCGGTGGGCAAATCTCTTCAGTTGCAGTAGTTCTGCAGCTC325380.12221446847579989No Hit
CTTCCTTATCAGCTCGGCATGATCTTTAAATGCAAAGTCAGCCACCTCAG321390.12071580313306694No Hit
CTGGCATCGGCGGTGGGCAAATCTCTTCAGTTACAGTAGTTCTGCAGCTC315270.11841709839684501No Hit
CTCAGTTTCCTGGGCAGATTTAGGAGGAAGGAAACCCAGAAGGGGCTCAA296080.11120923174846281No Hit
CGTTGAGCCCCTTCTGGGTTTCCTTCCTCCTAAATCTGCCCAGGAAACTG288210.10825321765139309No Hit
CTTTATCTGGTCTGTGGCCCAACAGGCTTCTTCTACAACCCCAAGAGAGA273630.10277689166215848No Hit
GAAGAAGCCTGTTGGGCCACAGACCAGATAAAGGGCATCGACCAGATGAG271390.10193553568027332No Hit
CTCAACGTCTCTCTTGGGGTTGTAGAAGAAGCCTGTTGGGCCACAGACCA268860.10098525414716196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATAT175100.019.2919141
CCATATC197100.017.7207032
CATATCC196450.017.7146663
ACGATCC40900.017.4798853
ACGCGTA36700.017.44253222
TATCCAC210150.016.643565
AGACGAT44500.016.3621441
ATATCCA219550.016.2716314
CGATCCA53300.016.0546534
CACGCGT42100.015.25752721
CGGGGTT39950.015.1328271
GAGTTAA89400.015.009663
ATTGGCG180900.014.7016566
CTTACCC80600.014.4812791
TGAGTTA97600.013.9131672
CCACGCG45700.013.91121420
CTGGCAT334850.013.8967291
CTGAGTT113800.013.8046061
GGTGGGC314950.013.5499812
CGGTGGG318600.013.36712811
Produced by FastQC (version 0.11.5)